期刊
VETERINARY JOURNAL
卷 191, 期 1, 页码 115-120出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.tvjl.2010.11.019
关键词
Tilmicosin; Solid lipid nanoparticles; Antibacterial activity; Therapeutic efficacy; Mastitis model
资金
- National High Technology Research and Development Program ('863' Program) of China [2006AA10A2030]
- China Agricultural University [kycx2010051]
- National Key Technology R and D Program [2006BAD04A02]
This study aimed to enhance the antibacterial activity of tilmicosin by solid lipid nanoparticles (SLN). Tilmicosin-loaded hydrogenated castor oil (HCO)-SLN was prepared using a hot homogenisation and ultrasonication method. The physicochemical characteristics of SLN were investigated by scanning electron microscopy (SEM) and photon correlation spectroscopy (PCS). The antibacterial activity of tilmicosin-SLN against Staphylococcus aureus was evaluated by growth inhibition and colony-counting method. A therapeutic study of tilmicosin-SLN was conducted by subcutaneous injection in a mouse mastitis model infected with S. aureus by teat canal infusion. Therapeutic efficacy was assessed by physical appearance of the mammary gland and measurement of colony-forming units (CFU) per gland. The results showed that the diameter, polydispersivity index, zeta potential, encapsulation efficiency and loading capacity of the nanoparticles were 343 +/- 26 nm, 0.33 +/- 0.08, -7.9 +/- 0.4 mV, 60.4 +/- 3.3% and 11.2 +/- 0.47%, respectively. Tilmicosin-SLN showed a sustained-release effect and sustained and enhanced antibacterial activity in vitro. SLN significantly enhanced the therapeutic efficacy of tilmicosin determined by lower CFU counts and a decreased degree of inflammation. These results demonstrated that the HCO-SLN is an effective carrier to enhance the antibacterial activity of tilmicosin. (C) 2010 Published by Elsevier Ltd.
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