期刊
VETERINARY JOURNAL
卷 194, 期 3, 页码 303-308出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.tvjl.2012.05.024
关键词
Canine; Lymphocyte subsets; Flow cytometry; Chemokines; gamma delta Lymphocytes
资金
- Ministry of Agriculture of the Czech Republic [MZE0002716202]
- Ministry of Education, Youth and Sports of the Czech Republic (AdmireVet) [CZ 1.05/2.1.00/01.0006, ED0006/01/01]
Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of gamma delta T lymphocytes was found in pyometra samples mainly due to recruitment of gamma delta(+)/CD8(-) T lymphocytes. The relative expression of genes encoding selected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1 beta, TNF-alpha, IL-8, IL-17 and IFN-gamma) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively. (C) 2012 Elsevier Ltd. All rights reserved.
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