期刊
VETERINARY JOURNAL
卷 181, 期 3, 页码 280-287出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.tvjl.2008.03.027
关键词
Horse; Respiratory explant; Morphometry; Viability
资金
- Institute for the promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)
An in vitro model of the upper respiratory tract of the horse was developed to investigate mechanisms of respiratory diseases. Four tissues of the upper respiratory tract of three horses were collected. Explants were maintained in culture at ail air-liquid interface for 96 h. At 0, 24, 48, 72 and 96 h of cultivation. a morphometric analysis was performed using light microscopy, scanning electron microscopy and transmission electron microscopy. The explants were judged oil morphometric changes of epithelium, basement membrane and connective tissue. Viability was evaluated using a fluorescent Terminal deoxynucleotidyl transferase-mediated dUTP Nick End Labelling (TUNEL) staining. No significant changes in morphometry and viability of any of the explants were observed during cultivation. Hence, the in vitro model may be useful to study infectious and non-infectious diseases at the level of the equine respiratory tract, with potential application to the development of vaccines and treatments for diseases of the respiratory tract. (C) 2008 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据