期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 203, 期 -, 页码 1-12出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2018.07.011
关键词
Turbot (Scophthalmus maximus); Immunoglobulin tau (IgT); Mucosal immune; Gene cloning; Gene expression response
资金
- National Natural Science Foundation of China [31730101, 31672685, 31672684, 31472295, 31302216]
- Taishan Scholar Program of Shandong Province
- Open Foundation of Functional Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology [2016LMFS-A01]
Immunoglobulins (Igs) are humoral mediators playing the prevailing role in the innate and adaptive immunities of jawed vertebrates and provide obligatory duties to protect the organism from a wide variety of pathogens. In the present study, the membrane-bound and secretory immunoglobulin T (mlgT and sIgT) genes of turbot (Scophthalmus maximus) were cloned for the first time with the intention of better understanding the IgT functions. The mIgT cDNA is 2, 049 bp in length including a leader region, a variable region, four constant regions and a transmembrane region (TM), while the 1, 932 bp slgT cDNA lacks the transmembrane region. In healthy turbot, the total IgT was highly expressed in gill, spleen and liver followed by peripheral blood leucocytes (PBL), skin and hindgut, and then in stomach, head kidney, trunk kidney, midgut and foregut. The expression levels of sIgT in PBL, gill, skin and spleen were much higher than mlgT. Furthermore, the expression profiles of turbot mlgT and slgT were investigated post vaccination with formalin-inactivated Vibrio anguillarum via intraperitoneal injection and immersion, and the results showed that the expressions of mlgT and sIgT were both significantly induced by two administration routes, whereas intraperitoneal injection mostly induced mlgT expression in systematic organs including head kidney, spleen and trunk kidney, and the immersion vaccination elicited a much stronger response of sIgT in mucosa-associated tissues including gills, liver, hindgut and skin. Taken together, these results demonstrated that mIgT and sIgT were differentially expressed in different tissues and both responded positively to the vaccinations in turbot, and indicated that IgT-secreting plasma cells are abundant in mucosa-associated tissues and played important roles in mucosal immunity of turbot.
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