期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 160, 期 1-2, 页码 41-50出版社
ELSEVIER
DOI: 10.1016/j.vetimm.2014.03.010
关键词
Rhodococcus equi; Macrophage; Foal; Pneumonia; Cytokines
资金
- Morris Animal Foundation [D10EQ-403]
Rhodococcus equi, a facultative intracellular pathogen of macrophages, causes life-hreatening pneumonia in foals and in people with underlying immune deficiencies. As a basis for this study, we hypothesized that macrophage lineage and age would affect intracellular survival of R. equi and cytokine induction after infection. Monocyte-derived and bronchoalveolar macrophages from 10 adult horses and from 10 foals (sampled at 1-3 days, 2 weeks, 1 month, 3 months, and 5 months of age) were infected ex vivo with virulent R. equi. Intracellular R. equi were quantified and mRNA expression of IL-1 beta, IL-4, IL-6, IL-8, IL-10, IL-12 p40, IL-18, IFN-gamma, and TNF-alpha was measured. Intracellular replication of It equi was significantly (P < 0.001) greater in bronchoalveolar than in monocyte-derived macrophages, regardless of age. Regardless of the macrophage lineage, replication of R. equi was significantly (P = 0.002) higher in 3-month-old foals than in 3-day old foals, 2-week-old foals, 1-month-old foals, and adult horses. Expression of IL-4 mRNA was significantly higher in monocyte-derived macrophages whereas expression of IL-6, IL-18, and TNF-alpha was significantly higher in bronchoalveolar macrophages. Induction of IL-1 beta, IL-10, IL-12 p40, and IL-8 mRNA in bronchoalveolar macrophages of 1-3-day old foals was significantly higher than in older foals or adult horses. Preferential intracellular survival of R. equi in bronchoalveolar macrophages of juvenile horses may play a role in the pulmonary tropism of the pathogen and in the window of age susceptibility to infection. (C) 2014 Elsevier B.V. All rights reserved.
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