期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 161, 期 3-4, 页码 132-140出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2014.07.006
关键词
Flow cytometry; Cancer; Stem cells; Melanoma; Osteosarcoma
资金
- National Center for Research Resources [5K01OD010911-04]
- Office of Research Infrastructure Progarms/OD
- Merck-Merial Summer Research Program
- Colorado State University Supercluster fund
- Shipley Foundation
Cancer stem cells (CSCs) represent a small subpopulation of tumor cells that play a critical role in initiating and sustaining tumor growth. However, we currently have an incomplete understanding of the expression patterns of CSC antigens in tumors of dogs, nor do we understand how expression of these antigens vary between tumor cell lines and tumor biopsy specimens. Therefore, we used flow cytometry and commonly reported CSC surface and intracellular markers to evaluate the phenotype and overall frequency of CSC subpopulations in tumor cell lines and primary tumor biopsy samples from dogs with melanoma and osteosarcoma. We found that cells expressing common CSC antigens were rare in tumor cell lines, with the exception of tumor cells expressing CD44 and CD90. In contrast, tumor cells expressing conventional CSC antigens such as CD133, CD34, CD44, CD24 and Oct3/4 were much more common in tumor biopsy samples. Notably, the frequency and types of putative CSC subpopulations were very similar in biopsy samples from dogs with either melanoma or osteosarcoma. Our results suggest that the tumor microenvironment significantly influences CSC subpopulations within tumors and that tumor cell lines may not accurately reflect the actual frequency or types of CSC subpopulations present in tumor tissues in vivo. (C) 2014 Elsevier B.V. All rights reserved.
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