期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 144, 期 3-4, 页码 228-237出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2011.09.004
关键词
Toll-like receptor; Chicken; Innate immunity; Cytokines; LPS; CpG
资金
- Ontario Ministry of Agriculture and Rural Affairs (OMAFRA) HQP
- Ontario Graduate Scholarship in Science and Technology
- Natural Sciences and Engineering Research Council of Canada
- Poultry Industry Council
- OMAFRA
Toll-like receptors (TLRs) are a group of conserved proteins that play an important role in pathogen recognition in addition to the initiation and regulation of innate and adaptive immune responses. To date, several TLRs have been identified in chickens, each recognizing different ligands. TLR stimulation in chickens has been shown to play a role in host-responses to pathogens. However, the mechanisms through which TLRs modulate the chicken immune system have not been well examined. The present study was conducted to characterize the kinetics of responses to TLR4 and TLR21 stimulation in chickens following intramuscular injections of their corresponding ligands, lipopolysaccharide (LPS) and CpG oligodeoxynucleotides (ODNs), respectively. To this end, relative expression of cytokine genes in the spleen was determined at 2, 6, 12 and 24h after injection of TLR ligands. The results indicated that LPS strongly induced the up-regulation of some immune system genes early on in the response to treatment, including interferon (IFN)-gamma, interleukin (IL)-10, and IL-1 beta. Furthermore, treatment with CpG ODN promoted the up-regulation of major histocompatibility complex (MHC)-II. IFN-gamma and IL-10. The response to CpG ODN appeared to be somewhat delayed compared to the response to LPS. Moreover, we found a significant increase in IFN-alpha gene expression in response to LPS but not CpG ODNs. Future studies may be aimed to further characterize the molecular mechanisms of TLR activation in chickens or to exploit TLR agonists as vaccine adjuvants. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据