期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 144, 期 1-2, 页码 95-103出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2011.07.007
关键词
Cytokines; Cell trafficking; Lymph node; Ovine
资金
- Australian Research Council
Lymphocyte trafficking from blood to lymph and back is a tightly regulated process. Given appropriate stimuli, trafficking of cells through the lymph node changes from a 'steady-state' to a bimodal flow. Initially, a 'shutdown' phase occurs, leading to a dramatic reduction in efferent cell output. This is followed by a 'recruitment' phase whereby the efferent cell output becomes greatly elevated before returning to baseline levels. The shutdown/recruitment process is hypothesised to promote encounters between Ag-specific lymphocytes and APCs in an environment conducive to immune response induction. Cytokines, such as TNF-alpha have been shown to play an important role in regulating lymphocyte trafficking. Here, we unravel the role of cytokines in the regulation of cell trafficking using an in vivo sheep lymphatic cannulation model whereby the prefemoral lymph nodes were cannulated and recombinant cytokines were injected subcutaneously into the draining area of the cannulated node. We demonstrate that local injection of purified IL-6 or TNF-alpha stimulates shutdown/recruitment in the draining lymph node. While the effect of IL-6 appears to be direct, TNF-alpha may mediate shutdown/recruitment through IL-6. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据