4.3 Article

Development of colloidal gold-based immunochromatographic assay for rapid detection of Streptococcus suis serotype 2

期刊

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 133, 期 2-4, 页码 207-211

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2009.08.010

关键词

Streptococcus suis serotype 2; Colloidal gold; Immunochromatography

资金

  1. National Natural Science Foundation of China [30870091, 30770117, 30600023]
  2. National Basic Research Program (973) of China [2006CB504400]
  3. High-Tech Research & Development Project 863 [2006AA02Z404, 2007AA02 1504]
  4. National Key Technologies RD program [2006BAD06A02]
  5. mega-projects of science research for the 11th Five-year plan [2009ZX10004-103]

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To develop colloidal gold immunochromatographic strips for the direct detection of the Streptococcus suis serotype 2 antigen, colloidal gold was prepared by reduction of a gold salt with sodium citrate and coupled with polyclonal antibody against S. suis serotype 2. The optimal concentrations of the capture antibody and the coating antibody were determined to be 22 mu g/mL and 2.0 mg/mL, respectively, and that of the blocking buffer was determined to be 1.5% bovine serum albumin. Different serotypes of S. suis and other related bacteria were used to evaluate the sensitivity, specificity, and stability of the immunochromatographic strips. The detection sensitivity was found to be as high as 10(6) CFU/mL There was no cross-reaction of the antibodies with other serotypes of S. suis (except with SS1/2, which shares some common sugar residues or antigenic determinants with serotype 2) and other related bacteria. In conclusion, we developed colloidal gold immunochromatographic strips that had high sensitivity and specificity. This method proved to be feasible, convenient, rapid, and effective for detecting S. suis serotype 2. (C) 2009 Elsevier B.V. All rights reserved.

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