期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 137, 期 1-2, 页码 20-27出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2010.04.005
关键词
Classical swine fever virus; Recombinant adenovirus; Alphavirus replicon; DNA vaccine; Prime-boost immunization
资金
- National 863 Projects of China [2006AA10A204]
Classical swine fever (CSF) - caused by the classical swine fever virus (CSFV) - is a fatal disease of pigs that is responsible for extensive losses to the swine industry worldwide We had demonstrated previously that a prime-boost vaccination strategy using an alphavirus (Semliki Forest virus, SFV) replicon-vectored DNA vaccine (pSFV1CS-E2) and a recombinant adenovirus (rAdV-E2) expressing the E2 glycoprotein of CSFV induced enhanced immune responses in a mouse model. In this study, we evaluated further the efficacy of the heterologous prime-boost immunization approach in pigs, the natural host of CSFV. The results showed that the pigs (n = 5) receiving pSFV1CS-E2/rAdV-E2 heterologous prime-boost immunization developed significantly higher titers of CSFV-specific neutralizing antibodies and comparable CD4(+) and CD8(+) T-cell proliferation, compared to the pigs receiving double immunizations with rAdV-E2 alone. When challenged with virulent CSFV Shimen strain, the pigs of the heterologous prime-boost group did not show clinical symptoms or viremia, which were observed in one of the 5 pigs immunized with rAdV-E2 alone and all the 5 control pigs immunized with an empty adenovirus The results demonstrate that the heterologous DNA prime and recombinant adenovirus boost strategy can induce solid protective immunity. (C) 2010 Elsevier B V All rights reserved
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