期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 132, 期 2-4, 页码 209-217出版社
ELSEVIER
DOI: 10.1016/j.vetimm.2009.06.009
关键词
Chicken; Immunology; CD4 T cell; CD8 T cell; Cytokine; Marek's disease; Real-time qRT-PCR; Disease resistance
资金
- Poultry Industry Council (PIC)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
T cells from the spleens of B-19/B-19 and B-21/B-21 chickens infected with MDV JM-16 strain were fractionated by flow cytometry at 4,10,21 days post infection (d.p.i.). The expression of cytokine and viral genes (meq and glycoprotein B (gB)) was measured by real-time RTPCR. It was determined that CD4(+) and CD8(+) T cells had both become infected with Marek's disease virus (MDV) in both chicken lines. There was significantly higher expression of meq in CD4(+) T cells compared to CD8(+) T cells at 10 and 21 d.p.i. Furthermore, at 10 and 21 d.p.i., there was a tendency for higher expression of meq in both T cell subsets of B-19 chickens compared to those of B-21 chickens. There were temporal changes in the expression of cytokines, interferon (IFN)-gamma, interleukin (IL)-18, IL-6, and IL-10, in various T cell subsets. Among these changes, there was an increase in IL-10 expression in both T cell subsets at different time points, especially in the susceptible line at 10 and 21 d.p.i. Our results indicate that cytokines could be differentially induced by MDV in CD4(+) and CD8(+) T cell subsets and that IL-10 may play a role in the modulation of immune response to MDV. However, an association between cytokine gene expression in T cell subsets and resistance or susceptibility to MID was not established. (C) 2009 Elsevier B.V. All rights reserved.
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