期刊
VECTOR-BORNE AND ZOONOTIC DISEASES
卷 12, 期 3, 页码 223-229出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/vbz.2011.0687
关键词
Flavivirus; Mosquitoes; Mosquito-only flavivirus
资金
- Spanish Health Ministry [G03=059]
- Red de Investigacion de Centros de Enfermedaes Tropicales (RICET) [RD06/0021]
- Institute of Health Carlos III
- Spanish Ministry of Health and Social Policy
- [FIS PI07/1308]
We report the characterization of three novel flaviviruses isolated in Spain. Marisma Mosquito virus, a novel mosquito borne virus, was isolated from Ochlerotatus caspius mosquitoes; Spanish Ochlerotatus flavivirus and Spanish Culex flavivirus, two novel insect flaviviruses, were isolated from Oc. caspius and Culex pipiens, respectively. During this investigation, we designed a sensitive RT-nested polymerase chain reaction method that amplifies a 1019bp fragment of the flavivirus NS5 gene and could be directly used in clinical or environmental samples for flavivirus characterization and surveillance. Analysis of the sequence generated from that amplicon contains enough phylogenetic information for proper taxonomic studies. Moreover, the use of this tool allowed the detection of additional flavivirus DNA forms in Culex, Culiseta, and Ochlerotatus mosquitoes.
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