期刊
VACCINE
卷 36, 期 41, 页码 6124-6132出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2018.08.068
关键词
Toxoplasma gondii; Multi-epitope; PLGA nanoparticle; Vaccine
资金
- Iran University of Medical Sciences [9221577201]
No effective human vaccine against Toxoplasma gondii (T. gondii) has yet been developed; however, a protective vaccine using immunogenic peptides in a safe delivery vehicle system offers promise. Here, we employed bioinformatics to design a multimeric recombinant T. gondii vaccine using predicted T and B cell epitopes of SAG1, AMA1, ROP2, and GRA4 proteins based on their binding capabilities to common major histocompatibility complex (MHC) molecules. Furthermore, we encapsulated the expressed protein in poly lactic-co-glycolic acid (PLGA) nanoparticles as a delivery vehicle and also used alum as an adjuvant to determine the vaccine potency of this multimeric antigen. BALB/c mice were vaccinated and then challenged with T. gondii RH strain, and the survival rate and cytokine profiles were studied. Mice vaccinated with the multi-epitope-based vaccine, both with and without PLGA, had greater Th1 immune responses, survival rates, specific antibody titers, and IFN-gamma and IL-2 levels than controls, while the alum-adsorbed vaccine stimulated a Th2-type humoral immune response. (C) 2018 Elsevier Ltd. All rights reserved.
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