期刊
VACCINE
卷 32, 期 15, 页码 1693-1699出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2014.01.064
关键词
Disorganized muscle protein-1; Brugia malayi; Macrophage activity; IFN-gamma; IgG and its subclasses; Nitric oxide
资金
- Network project (SPLENDID) of Council of Scientific and Industrial Research, New Delhi, India
- Indian Council of Medical Research and Department of Biotechnology, New Delhi, India
We have recently identified disorganized muscle protein-1 (DIM-1) in one of the proinflammatory fractions of the human filaria Brugia malayi adult worm. The present study was undertaken to characterize B. malayi DIM-1 (DIM-1bm) and explore its vaccine potential. In this study we cloned and expressed the DIM-1bm gene, investigated its sequence homology with other nematodes, constructed in silico structural model, purified the recombinant DIM-1bm (rDIM-1bm) protein, and studied the effect of immunization with rDIM-1bm on the establishment of B. malayi infection in Mastomys coucha. DIM-1bm showed similarity with DIM-1 of Caenorhabditis elegans, Ascaris suum and Loa loa. Structural modeling revealed three immunoglobulin domains in DIM-1bm indicating that it is a member of immunoglobulin superfamily (IgSF) and 'blastn' results showed that DIM-1bm coding sequence (CDS) have almost no homology with human and mouse nucleotide sequences. Immunization with rDIM-1bm partially protected M. coucha against establishment of infection as inferred by a low recovery of microfilariae (37-64%) and parasite burden (similar to 50%). The enhanced activity of macrophages, and IFN-gamma and NO responses, and elevated levels of specific IgG, IgG1, IgG2a and IgG2b correlated with parasitological findings. This is the first report on cloning, expression, structural modeling and purification of rDIM-1bm and its ability to partially prevent establishment of B. malayi infection. DIM-1bm's almost complete lack of homology with the human counterpart makes it an attractive protein for exploring its vaccine potential. (C) 2014 Elsevier Ltd. All rights reserved.
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