期刊
VACCINE
卷 32, 期 16, 页码 1808-1813出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2014.01.088
关键词
Olive flounder; Pseudomonas anguilliseptica; Winter diseases; Jeju Island; Immune gene expression
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education (MOE) through the Human Resource Trainning Project [2013HIB8A2032163]
- Fisheries Research Institute, Pyoseon-myeon, Segwipo-si, Jeju South Korea [697-914]
Formalin killed Pseudomonas anguilliseptica bacterial vaccine was prepared and administered to farm reared olive flounder, Paralichthys olivaceus reared at 17 degrees C and 20 degrees C for 4 weeks. Non-vaccinated fishes (n = 150) served as positive control. Vaccinated fishes were divided into two groups (n = 150 each in replicate). Both the vaccinated and non-vaccinated fishes were challenged intraperitoneally with P. anguilliseptica (1 x 10(7) CPU ml(-1)) isolates and PBS (negative control). Fishes were sampled from zero hour post injection (hpi) for 28 days (each hour and each day); the mean percent mortality and relative percent survival (RPS) were calculated for the challenged and control groups. The vaccinated fishes had a significant increase in RPS (69 and 89, respectively); the percentage mortality declined from 83 +/- 0.6 and 74 +/- 0.7 in challenged and control fishes to 25% +/- 0.8% and 8% +/- 0.8% in vaccinated and challenged fish groups, respectively. The immune gene expression assay was analyzed using real-time PCR. Vaccinated fishes registered a significant increase in the expression of TNFR-1, FasL, IRF7, TLR2, IL-1b and CD40 gene transcripts when compared to the control group. The upregulation of these genes along with the increased RPS values suggest that the formalin-killed cells of P.anguilliseptica could play an important role in immunizing olive flounder against P.anguillisepdca. (c) 2014 Elsevier Ltd. All rights reserved.
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