期刊
VACCINE
卷 32, 期 35, 页码 4578-4585出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2014.06.040
关键词
Influenza virus; M2e VLPs; Adjuvants; C57BL/6
资金
- NIH/NIAID [AI105170, AI093772]
- Animal and Plant Quarantine Agency
The ectodomain of matrix protein 2 (M2e) of influenza virus is suggested to be a rational target for a universal influenza A vaccine. However, there are some concerns that M2e vaccines might not be highly effective in the general population with diverse genetic backgrounds. Here we examined the immuno-genicity and protective efficacy of the baculovirus-derived virus-like particles containing multiple M2e (M2eVLP) with AS04 adjuvant in a C57BL/6 mouse strain (H-2(b)). M2eVLP vaccine induced significant levels of M2e-specific IgG in C57BL/6 mice after vaccination. Furthermore, M2eVLP adjuvanted with AS04 was more effective than M2eVLP alone in conferring protection as well as in inducing recall humoral and T cell responses specific for M2e after lethal influenza virus challenge. A mechanistic study provides evidence that activation of dendritic cells by the toll-like receptor 4 agonist MPL in the AS04 adjuvant was associated with interferon-gamma producing CD4 T cell responses. Our results suggest that AS04 adjuvanted M2eVLP vaccines have the potential to improve cross-protection. (C) 2014 Elsevier Ltd. All rights reserved.
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