期刊
VACCINE
卷 32, 期 46, 页码 6034-6038出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2014.08.060
关键词
S19; RB51; Calfhood vaccination; Revaccination of pregnant cattle; Cellular immunity; Brucellosis
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (Fapemig)
- FEP-MVZ Coordenacao Preventiva
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq
- CNPq
- Capes (Ciencia Sem Fronteiras) [8808-13]
The aims of this study were to address the protective immune response induced by S19 vaccination (n = 10) and RB51 revaccination, in pregnant (n = 9) and non-pregnant (n = 10) S19 calfhood-vaccinated cattle as follows: evaluate the in vitro CD4(+) and CD8(+) T-lymphocytes specific proliferation, and in vitro expression of IFN-gamma by CD4(+) and CD8(+) T-cells and IL-4 by CD4(+), CD8(+) and CD21(+) lymphocytes subset. Upon in vitro stimulation with gamma-irradiated Brucella abortus 2308, blood mononuclear cells from S19 vaccinated and RB51 revaccinated cows exhibited significantly higher proliferation of CD4(+) and CD8(+) T-lymphocytes and CD4(+)IFN-gamma(+) T-cells compared to non-vaccinated animals. RB51 revaccination, regardless of the pregnancy status, did not enhance the proliferation of CD4(+) or CD8(+) T-cells nor IFN-gamma or IL-4 production. Data from the present study suggest that cattle's cellular immune response induced after brucellosis vaccination and revaccination is due to CD4(+) and CD8(+) T-lymphocytes, being CD4(+) T-cells the main source of IFN-gamma. (C) 2014 Published by Elsevier Ltd.
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