期刊
VACCINE
卷 32, 期 3, 页码 401-408出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2013.11.027
关键词
Vaccine; Adjuvant; Ganodemia formosanum; Polysaccharides; Th1 response; CTL response
资金
- National Science Council [NSC99-2628-B-002-005-MY3]
- Council of Agriculture, Taiwan [98AS-3.1.3-FD-Z1]
- National Health Research Institutes [NHRI-EX97-9724SC]
- National Taiwan University, Taiwan [NTU-CDP-102R7824]
The fungus of Ganoderma is a basidiomycete that possesses a variety of pharmacological effects and has been used in traditional Asian medicine for centuries. Ganoderma formosanum is a native Ganoderma species isolated in Taiwan, and we have previously demonstrated that PS-F2, a polysaccharide fraction purified from the submerged culture broth of G. formosanum, exhibits immunostimulatory properties in macrophages. In this study, we further characterized the adjuvant functions of PS-F2. In vitro, PSF2 stimulated dendritic cells (DCs) to produce proinflammatory cytokines, including TNF-alpha, interleukin (IL)-6, and IL-12/IL-23 p40. PS-F2 also stimulated DCs to express the maturation markers CD40, CD80, CD86, and MHC class II. In a murine splenocyte culture, PS-F2 treatment resulted in elevated expression of T-bet and interferon (IFN)-gamma in T lymphocytes. When used as an adjuvant in vivo with the ovalbumin (OVA) antigen, PS-F2 stimulated OVA-specific antibody production and primed IFN-gamma production in OVA-specific T lymphocytes. PS-F2-adjuvated immunization also induced OVA-specific CTLs, which protected mice from a challenge with tumor cells expressing OVA. Collectively, our data show that PS-F2 functions as an adjuvant capable of inducing a Th1-polarized adaptive immune response, which would be useful in vaccines against viruses and tumors. (C) 2013 Elsevier Ltd. All rights reserved.
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