期刊
VACCINE
卷 31, 期 2, 页码 367-372出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2012.10.107
关键词
Stx2eB; FedF; Escherichia coli; Edema disease
资金
- MATS-Beef Cattle Yak system [CARS-38]
- Chinese Academy of Science Knowledge Innovation Project [KZCX2-EW-QN411, KSCX2-YW-N-051]
- Planned Science and Technology Project of Hunan Province [2012WK4021]
- Program of State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences [SKLMR-20110607]
Piglet edema disease is found worldwide and has historically been treated with antibiotics. However, no commercial vaccines are available for its prevention. In this study, the two major virulence factors of Shiga-toxigenic Escherichia coli (Stx2eB and FedF) were cloned to a pcDNA6.0 plasmid to develop a novel DNA vaccine against piglet edema disease. In animal trial in mouse model, the antibody titer, mortality, serum cytokine levels (interleukin-1 beta, interleukin-6, tumor necrosis factor alpha and C-reactive protein), serum malondialdehyde level and serum total superoxide dismutase activity were measured to validate the effectiveness of the DNA vaccine. The results show that Stx2eB and FedF at least partially protect against edema disease and FedF is more effective than Stx2eB. Co-immunization with both Stx2eB and FedF is most effective for protecting mice from a subsequent challenge with E. coli 0139 (which is known to cause edema disease in pigs). (C) 2012 Elsevier Ltd. All rights reserved.
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