期刊
VACCINE
卷 30, 期 10, 页码 1782-1789出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2012.01.011
关键词
Tick; Vaccination; P0 protein; Peptide immunization
资金
- Center for Genetic Engineering and Biotechnology, Havana, Cuba
Ticks are acaridae ectoparasites that, while taking a blood meal, can transmit viruses, bacteria, protozoa and filarial nematodes, which cause a variety of human and animal illnesses. The use of chemical pesticides constitutes the primary measure for control of these ectoparasites. However, the intensive use of these chemicals has drawbacks such as the contamination of food, environmental pollution and development of resistance by ectoparasites. Vaccination is considered a promising alternative for controlling infestations by ectoparasites. Although emerging tick proteins have been identified recently, and have been proposed as potential targets for generating protective molecules, only a limited number of them have been evaluated in vaccine trials. More than 80 proteins are found in eukaryotic ribosomes. The protein PO is essential for the assembly of the 60S ribosomal subunit. We have identified an immunogenic region of the ribosomal protein PO from Rhipicephalus sp. ticks that is not very conserved compared to host PO. The efficacy of a 20 amino acid synthetic peptide from this sequence was assayed as a vaccine antigen against Rhipicephalus sanguineus infestations in an immunization and challenge experiment on rabbits. A remarkable diminution in the viability of newly molted nymphs from larvae fed on vaccinated rabbits was observed. The number of adults and the number of eggs hatching were significantly reduced, with an overall efficacy of 90%. Our results demonstrated that immunization with an immunogenic peptide of tick protein PO greatly reduced survival of ticks, suggesting that it has promise as an effective tick control agent. (C) 2012 Elsevier Ltd. All rights reserved.
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