期刊
VACCINE
卷 30, 期 23, 页码 3478-3482出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2012.02.075
关键词
Ascaris suum; Enolase; Eukaryotic expression; Protective immunity; Vaccine
资金
- National Natural Science Foundation of China [30671578, 30901067]
- Program for Outstanding Scientists in Agricultural Research
- State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences [SKLVEB2009KFKT014, SKLVEB2010KFKT010, SKLVEB2010KFKT009, SKLVEB2011KFKT004, SKLVEB2011KF-KT010, SKLVEB2011KFKT011]
- Yunnan Provincial Program for Introducing High-level Scientists [2009CI125]
Ascariasis caused by Ascaris is the most common parasite problem in humans and pigs worldwide. No vaccines are available for the prevention of Ascaris infections. In the present study, the gene encoding Ascaris suum enolase (As-enol-1) was amplified, cloned and sequenced. Amino acid sequence alignment indicated that As-enol-1 was highly conserved between different nematodes and shared the highest identity (87%) with enolase from Anisakis simplex s.l. The recombinant pVAX-Enol was successfully expressed in Marc-145 cells. The ability of the pVAX-Enol for inducing immune protective responses against challenge infection with A. suum L3 was evaluated in Kunming mice. The immune response was evaluated by lymphoproliferative assay, cytokine and antibody measurements, and the reduction rate of recovery larvae. The results showed that the mice immunized with pVAX-Enol developed a high level of specific antibody responses against A. suum, a strong lymphoproliferative response, and significant levels of IFN-gamma, IL-2, IL-4 and IL-10 production, compared with the other groups immunized with empty plasmid or blank controls, respectively. There was a 61.13% reduction (P < 0.05) in larvae recovery compared with that in the blank control group. Our data indicated that A. suum enolase is a potential vaccine candidate against A. suum infection. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.
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