期刊
VACCINE
卷 30, 期 34, 页码 5067-5072出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2012.06.001
关键词
Rabies; Canine distemper; Recombinant canine distemper virus; Vaccine
资金
- Chinese National ST Plan [2012ZX10004214]
- Chinese Ministry of Agriculture [201103032]
- Fundamental Researching Funds for central public welfare research institutes [03020120004]
Effective, safe, and affordable rabies vaccines are still being sought. Attenuated live vaccine has been widely used to protect carnivores from canine distemper. In this study, we generated a recombinant canine distemper virus (CDV) vaccine strain, rCDV-RVG, expressing the rabies virus glycoprotein (RVG) by using reverse genetics. The recombinant virus rCDV-RVG retained growth properties similar to those of vector CDV in Vero cell culture. Animal studies demonstrated that rCDV-RVG was safe in mice and dogs. Mice inoculated intracerebrally or intramuscularly with rCDV-RVG showed no apparent signs of disease and developed a strong rabies virus (RABV) neutralizing antibody response, which completely protected mice from challenge with a lethal dose of street virus. Canine studies showed that vaccination with rCDV-RVG induced strong and long-lasting virus neutralizing antibody responses to RABV and CDV. This is the first study demonstrating that recombinant CDV has the potential to serve as bivalent live vaccine against rabies and canine distemper in animals. (C) 2012 Elsevier Ltd. All rights reserved.
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