期刊
VACCINE
卷 28, 期 39, 页码 6445-6454出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2010.07.045
关键词
BVDV E2 protein; Newborn calves; DNA vaccine; Electroporation
资金
- Alberta Beef Producers
- Alberta Livestock Industry Development Fund
- Saskatchewan Agricultural Development Fund
- Alberta Livestock and Meat Agency
- Agriculture and Food Council of Alberta
Bovine viral diarrhea virus (BVDV) is one of the major pathogens in cattle. In this study, newborn calves with maternal antibodies were vaccinated with a BVDV DNA vaccine, either by conventional intramuscular (IM) injection or with the TriGrid (TM) Delivery System for IM delivery (IDS-IM). The calves vaccinated with the TDS-IM developed more rapidly and effectively BVDV-specific humoral and cell-mediated immune responses in the presence of maternal antibodies. Overall, the immune responses induced by delivery with the TDS-IM remained stronger than those elicited by conventional IM injection of the BVDV DNA vaccine. Accordingly, electroporation-mediated delivery of the BVDV DNA vaccine resulted in close to complete protection from clinical signs of disease, while conventional IM administration did not fully prevent morbidity and mortality following challenge with BVDV-2. These results demonstrate the TDS-IM to be effective as a delivery system for a BVDV DNA vaccine in newborn calves in the presence of maternal antibodies, which supports the potential of electroporation as a delivery method for prophylactic DNA vaccines. (C) 2010 Elsevier Ltd. All rights reserved.
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