期刊
VACCINE
卷 28, 期 31, 页码 5038-5048出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2010.05.012
关键词
Filariasis; Multi-epitope vaccine; Thioredoxin
资金
- Department of Biotechnology (DBT), Govt. of India
- Indian Council Of Medical Research (ICMR)
- University Grants Commission (UGC)
Although multi-epitope vaccines have been evaluated for various diseases, they have not yet been investigated for lymphatic filariasis. Here, we report for the first time identification of two immunodominant B epitopes (TRXP1 and TRXP2) from the antioxidant Brugia malayi thioredoxin by studying their immune responses in mice model and human subjects. TRXpi was also found to harbor a T epitope recognized by human PBMCs and mice splenocytes. Further, the epitopic peptides were synthesized as a single peptide conjugate (PC1) and their prophylactic efficacy was tested in a murine model of filariasis with L3 larvae. PC1 conferred a significantly high protection (75.14%) (P < 0.0001) compared to control (3.7%) and recombinant TRX (63.03%) (P < 0.018) in experimental filariasis. Our results suggest that multi-epitope vaccines could be a promising strategy in the control of lymphatic filariasis. (C) 2010 Elsevier Ltd. All rights reserved.
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