期刊
VACCINE
卷 28, 期 47, 页码 7483-7491出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2010.09.014
关键词
DNA vaccine; Microprojection; Herpes simplex virus; Skin
资金
- PATH
- Australian Research Council
- Queensland Smart State Scheme
- Bill and Melinda Gates Foundation
There is an unmet medical need for a prophylactic vaccine against herpes simplex virus (HSV). DNA vaccines and cutaneous vaccination have been tried for many applications, but few reports combine this vaccine composition and administration route. We compared DNA administration using the Nanopatch (TM). a solid microprojection device coated with vaccine comprised of thousands of short (110 mu m) densly-packed projections (70 mu m spacing), to standard intramuscular DNA vaccination in a mouse model of vaginal HSV-2 infection. A dose-response relationship was established for immunogenicity and survival in both vaccination routes. Appropriate doses administered by Nanopatch (TM) were highly immunogenic and enabled mouse survival. Vaginal HSV-2 DNA copy number day 1 post challenge correlated with survival, indicating that vaccine-elicited acquired immune responses can act quickly and locally. Solid, short, densely-packed arrays of microprojections applied to the skin are thus a promising route of administration for DNA vaccines. (C) 2010 Published by Elsevier Ltd.
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