期刊
VACCINE
卷 27, 期 41, 页码 5603-5611出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.07.029
关键词
Porcine reproductive and respiratory syndrome virus; T-cell epitopes; Bioinformatics
资金
- Spanish Ministry of Science and Innovation [AGL2005-07073-C02-02/GAN, CDS2006-00007]
T-cell epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) glycoproteins 4 (GP4), 5 (GP5) and nucleocapsid (N) were predicted using bioinformatics and later tested by IFN-gamma ELISPOT in pigs immunized with either a modified live vaccine (MLV) or DNA (open reading frames 4, 5 or 7). For MLV-vaccinated pigs, immunodominant epitopes were found in N but T-epitopes were also found in GP4 and GP5. For DNA-immunized pigs, some peptides were differently recognized. Using a large set of PRRSV sequences it was shown that N contains a conserved epitope and that for GP5, the genotype-I counterparts of previously reported epitopes of genotype-II strains were also immunogenic. (c) 2009 Elsevier Ltd. All rights reserved.
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