期刊
VACCINE
卷 27, 期 38, 页码 5271-5277出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.06.051
关键词
Haemophilus parasuis; Immunoproteomics; Outer membrane proteins; Vaccine candidate
资金
- 973 program [2006CB504404]
- 863 program [2006AA10A206]
- National Key Technology RD Program [2006BAD06A11]
Haemophilus parasuis is the aetiological agent of Glasser's disease, which is responsible for cases of fibrinous polyserositis, polyarthritis and meningitis in young pigs. To develop more effective vaccines, an immunoproteome-based approach was used to analyze the outer membrane proteins of H. parasuis serovar 5. A total of 15 proteins with high immunogenicity were identified and all were showed to be immunogens for the first time in H. parasuis. Further analyses of 8 selected proteins revealed that (I) significantly higher level of serum antibodies against 6 proteins was detected with convalescent sera and immunized sera: (2) antisera against 5 of the selected proteins could effectively inhibit H. parasuis growth in mouse blood: and (3) 4 proteins could induce protective response of the vaccinated mice against H. parasuis. The results suggest these 4 proteins (PalA, Omp2, D15 and HPS-06257) have strong potential to be vaccine candidates. (C) 2009 Elsevier Ltd. All rights reserved.
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