期刊
VACCINE
卷 27, 期 45, 页码 6270-6275出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.05.053
关键词
Influenza; Packaging signal; Nucleoprotein
资金
- Medical Research Council [G0700815] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- MRC [G0700815] Funding Source: UKRI
- Medical Research Council [G0700815] Funding Source: researchfish
Influenza A virus vRNA segments contain specific packaging signals at their termini that overlap the coding regions. To further characterise segment 5 packaging signals, we introduced synonymous mutations into the terminal coding regions of the vRNA and characterised the replicative fitness of the resulting viruses. Most mutations tested were well-tolerated, but a virus with alterations to NP codons 464-466, near the 5'-end of the vRNA, produced small plaques and replicated to around one-tenth of the level of wild type virus. The mutant virus supported normal levels of NP and segment 5 vRNA synthesis but packaged reduced levels of both segment 5 and segment 3 into virus particles. This Suggests an interaction between segments 3 and 5 during influenza A virus assembly. (C) 2009 Elsevier Ltd. All rights reserved.
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