期刊
VACCINE
卷 27, 期 37, 页码 5103-5110出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.06.041
关键词
HAdV; Hexon protein; Epitope Mapping
The hexon protein of human adenovirus (HAdV) processes type-specific B-cell neutralizing epitopes. We developed a new effective, reliable approach to map these epitopes on hexon protein of HAdVs. A three-dimensional (3D) model of the HAM hexon was obtained by homology modeling and refined by molecular mechanics and molecular dynamics simulations. A modified evolutionary trace (ET) analysis called reverse ET (RET) was used to predict the type-specific B-cell neutralizing epitopes. An epitopescreening algorithm based on analyzing the solvent accessibility surface (SAS) area from the 31) model and calculation of sites homology using RET was designed and implemented in the BioPerl script language. Five epitope polypeptide segments were predicted and mapped onto the 31) model. Finally five polypeptides were synthesized and the predicted epitopes were identified by enzyme-linked immunosorbent assay (ELISA) and Neutralization Test (NT). It was found that the type-specific neutralizing epitopes of HAdV3 are located at the top surface of hexon tower regions (residue numbers: 135-146, 169-178, 237-251, 262-272, 420-434). This work is of great significance to the molecular design of a multivalent HAdVs vaccine. (C) 2009 Elsevier Ltd. All rights reserved.
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