期刊
VACCINE
卷 27, 期 9, 页码 1289-1292出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2008.12.050
关键词
Plant biotechnology; Avian flu H5N1; Recombinant subunit vaccine
资金
- Commonwealth of Pennsylvania Department of Health to Biotechnology Foundation
Polypeptide variants of the HA1 antigenic domain of the H5N1 avian influenza virus hemagglutinin (HA) molecule were produced ill plants using transient and stable expression systems and fused with His/c-myc tags or with mouse or human Fc antibody fragments. The resulting peptides were purified and used for intramuscular immunization of mice. While the recombinant HA1 variants induced a significant serum humoral immune response in the mice, none of the HA1 preparations induced virus-neutralizing antibodies. Fusion with the Fc fragment improved overall yield of the constructs and allowed purification requiring only a single step, but led to no detectable fusion-related enhancement of immunogenicity or quality of immune response. (C) 2009 Elsevier Ltd. All rights reserved.
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