期刊
VACCINE
卷 27, 期 41, 页码 5565-5573出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.07.035
关键词
Cytotoxic T lymphocytes; Vaccination; Trogocytosis
资金
- ANR RIB
- ITAV
- Region Midi-Pyrenees
- CNRS
Vaccination with recombinant adenylate cyclase of Bordetella pertussis (CyaA) carrying antigen is a promising approach to target antigen-presenting cells. We have used Trogocytosis Analysis Protocol (TRAP) assays to monitor immune responses raised by different vaccination regimens with recombinant CyaA carrying the ovalbumin antigen. We find that the intradermal, intramuscular or subcutaneous routes are all superior to intravenous injections, and actually lead to a sufficiently high frequency of reactive CTL to be detected and characterized directly ex vivo by TRAP assay or other standard assays. Finally. for all routes, we find a clear boosting effect upon re-injection of the vaccine. (c) 2009 Elsevier Ltd. All rights reserved.
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