期刊
VACCINE
卷 26, 期 29-30, 页码 3608-3616出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2008.04.074
关键词
human papillomavirus; neutralization assay; cervical secretions
资金
- Intramural NIH HHS [ZIA CP010217-3] Funding Source: Medline
- NCI NIH HHS [N01CO12400, N01-CO-12400, N01CP11005] Funding Source: Medline
Ideal methods to monitor HPV neutralizing antibodies induced by vaccination have not been established yet. Here, we evaluated systemic and cervical antibody levels induced by HPV16/18 AS04-adjuvanted vaccine (GlaxoSmithKline Biologicals) using a secreted alkaline phosphatase neutralization assay (SEAP-NA) and enzyme-linked immunosorbent assay (ELISA). Serum and cervical secretions from 50 vaccinated women were used to assess (1) overall assay reproducibility; (2) inter-assay and inter-specimen correlation; (3) correlations between month I and month 12 titers. Strong correlations between SEAP-NA and ELISA were observed (serum anti-HPV16/18, rho = 0.91/0.85; cervix anti-HPV16/18, rho = 0.84/0.89). Systemic and cervical antibody measures also correlated well (rho range: 0.64-0.75); except at mid-cycle (rho range: 0.28-0.65). Correlations between antibody levels at 1 and 12 months following the start of vaccination were poor (rho range: 0.16-0.38). In conclusion, HPV16/18 VLP-based ELISA is a reliable and valid method to monitor anti-HPV16/18 neutralizing potential for the first year following vaccination; however, additional studies will be required to better define the effects of the time on cycle and patterns of antibody response over time following vaccination. (C) 2008 Elsevier Ltd. All rights reserved.
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