Genetic Association of Monocyte Chemoattractant Protein 1 (MCP-1)-2518 Polymorphism in Mexican Patients With Transitional Cell Carcinoma of the Bladder

OBJECTIVE To investigate the role of MCP-1-2518, stromal cell-derived factor-1 (SDF-I)-G801A and chemokine receptors CCR5-Delta 32, CCR5-59 029, and CCR2-641 gene polymorphisms in transitional cell carcinoma of the bladder in Mexican Mestizo patients. METHODS Forty-seven unrelated consecutive patients with non-muscle-invasive transitional cell carcinoma (TCC) and 126 unrelated healthy individuals were studied. Genomic extraction was carried out from complete blood samples using the salting out method. The PCR-RFLP method was used to amplify the following polymorphisms: MCP-1-2518, SDF-1-G801A, CCR5-Delta 32, CCR5-59 029, and CCR2-641. RESULTS The patients were divided according to low, intermediate, and high risk of progression and treated accordingly with surveillance or immunotherapy with BCG. SDF-1-GS01A, CCR5-Delta 32, CCR5-59 029, and CCR2-641 polymorphisms were not associated with the genetic susceptibility to non-muscle-invasive TCC of the bladder in Mexican patients. The distribution of AA, AG, and GG genotypes of MCP-1-2518 was significantly different in bladder cancer patients compared with controls (P =.006). There was a significant decrease both in the frequency of the G mutant allele (P =.021, OR = 1.752; C 95% CI 1.088-2.828) and in the GG genotype (P=.001, OR = 6.097 95% Cl 1.885-19.570) in TCC patients, compared with controls. CONCLUSIONS This preliminary study shows that MCP-1 polymorphism is associated with TCC. UROLOGY 74: 414-418, 2009. (C) 2009 Elsevier Inc.