期刊
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
卷 26, 期 6, 页码 634-640出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.urolonc.2007.07.016
关键词
Real-time quantitative RT-PCR; Prostate-specific antigen (PSA); Prostate-specific membrane antigen (PSMA); Prostate cancer (PCa); Micrometastasis
Objective: Reverse transcription-polymerase chain reaction (RT-PCR) was, widely Used to detect disseminated tumor cells. However, high percentages of false-positive cases have been reported. Herein, we developed a method to detect prostate cancer (PCa) micrometastasis by real-time quantitative RT-PCR. Methods: Blood samples were obtained from 118 males, including, 13 healthy volunteers, 17 patients with metastatic PCa, 20 patients with localized PCa, and 68 patients with BPH. RNA was isolated from blood samples of different patients. Real-time RT-PCR was used to quantify file copy number of PSA and PSMA. The primers and probe of PSMA were designed to specifically discriminate between PSMA and PSM', an alternatively spliced variant. mRNA. Results: There was significant difference in the PSA and PSMA mRNA levels among BPH, locally confined PCa, and metastatic PCa blood specimens, The real-time quantitative RT-PCR is sensitive, accurate, and has a high reproducibility within a wide dynamic range (10(3)-10(8)), which permits simultaneous quantitative analysis of samples With varying input Concentrations. Conclusions: This method improves accuracy and liability of assessment of disseminated prostate cancer cells in peripheral blood and is promising for clinical diagnosis purpose. (c) 2008 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据