期刊
BIOTECHNOLOGY JOURNAL
卷 10, 期 9, 页码 1360-1369出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201400422
关键词
Gene expression; Metabolic engineering; Metabolite sensors; Synthetic biology
资金
- US National Science Foundation through the CAREER program [CBET-0954986]
- US National Science Foundation through the Biotechnology Training Program of the National Institutes of Health [T32GM008334]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0954986] Funding Source: National Science Foundation
Metabolic engineering strategies have enabled improvements in yield and titer for a variety of valuable small molecules produced naturally in microorganisms, as well as those produced via heterologous pathways. Typically, the approaches have been focused on up-and downregulation of genes to redistribute steady-state pathway fluxes, but more recently a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium. This review highlights some of the recently published work related to dynamic metabolic engineering strategies and explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems. Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates. The implementation is more complex relative to static control, but advances in screening techniques and DNA synthesis will continue to drive innovation in this field.
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