期刊
ULTRASOUND IN MEDICINE AND BIOLOGY
卷 40, 期 9, 页码 2172-2182出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ultrasmedbio.2014.03.018
关键词
High-frequency ultrasound microbeam; Mechanotransduction; Human umbilical vein endothelial cells; Calcium fluorescence imaging
资金
- National Institutes of Health
- United States of America [R01-EB012058, P41-EB002182]
- International Collaborative R&D Program - Ministry of Trade, Industry & Energy (MOTIE), Korea [N01150049]
- Daegu Gyeongbuk Institute of Science Technology
- Ministry of Trade, Industry & Energy (MOTIE), Republic of Korea [N0000684] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2014R1A1A2054934] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechano-transduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. Avariety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified. (E-mail: jyhwang@dgist.ac.kr) (C) 2014 World Federation for Ultrasound in Medicine & Biology.
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