期刊
ULTRAMICROSCOPY
卷 110, 期 1, 页码 43-47出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultramic.2009.09.004
关键词
Cryo-section; Section resolution
类别
资金
- Wellcome Trust (UK)
- Overseas Research Scholarship (UK)
- Medical Research Council [MC_U105184294] Funding Source: researchfish
- MRC [MC_U105184294] Funding Source: UKRI
We have quantitated the degree of structural preservation in cryo-sections of a vitrified biological specimen. Previous studies have used sections of periodic specimens to assess the resolution present, but preservation before sectioning was not assessed and so the damage due particularly to cutting was not clear. In this study large single crystals of lysozyme were vitrified and from these X-ray diffraction patterns extending to better than 2.1 angstrom were obtained. The crystals were high pressure frozen in 30% dextran, and cryo-sectioned using a diamond knife. In the best case, preservation to a resolution of 7.9 angstrom was shown by electron diffraction, the first observation of sub-nanometre structural preservation in a vitreous section. (C) 2009 Elsevier B.V. All rights reserved.
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