Effect of Exposure Duration on Time to Recovery from Anaesthesia of Clove Oil in Juvenile of Russian Sturgeon

The efficacy of clove oil as an anaesthetic was evaluated in Juvenile of Russian sturgeon (Acipenser gueldenstaedtii) under two experiments. Dose response examined using four anaesthetic concentrations varying from 0.22 to 0.90 g/L of clove oil and effect of exposure duration on the recovery time analysed for 5 and 10 min. Mean body length (cm), 24.9 +/- 1.37 and weight (g) 63.2 +/- 10.89, n=120, juvenile Russian sturgeon placed individually to the anaesthetic bath prepared with clove oil, and induction (II: Sedation and 12: Loss of equilibrium) and recovery times (RI: Regain of equilibrium and R2: Sedation) recorded in seconds. Allowing fish remain in the anaesthesia after fish reaching anaesthesia stage, time to recovery from anaesthesia recorded. Fish exposed to clove oil failured to respond to external stimuli and reached to anaesthesia, sympocal stage in <3 min and recovered in <5 min at all doses except 0.22 g/L dose of clove oil. Exposure five and ten minute to anaesthesia at the 0.22, 0.45 and 0.90 g/L concentrations, fish recovered from anaesthesia in <10 min, except 0.90 g/L. The higher dose of clove oil resulted in significantly shorter induction time and prolonged recovery time. While correlation between induction time and dose determined as negative, r= -0.594 and a positive correlation has been found between dose and recovery time, r=0.422 (P<0.05). The study demonstrated that clove oil can be used as an effective anaesthetic measuring length and weight of juvenile Russian sturgeon and dose and exposure duration effect on time to recovery from anaesthesia of clove oil.