期刊
TUMOR BIOLOGY
卷 35, 期 2, 页码 1377-1382出版社
SAGE PUBLICATIONS LTD
DOI: 10.1007/s13277-013-1188-y
关键词
ILEI; Lung cancer; Metastasis; ? Epithelial-mesenchymal transition; Cancer stem cells
类别
Transforming growth factor beta (TGF-beta) induces epithelial-mesenchymal transition (EMT) accompanied by cellular differentiation and migration. Despite extensive transcriptomic profiling, identification of TGF-beta-inducible, EMT-specific genes during metastatic progression of lung cancer remains elusive. Here, we functionally validate a previously described post-transcriptional pathway by which TGF-beta modulates expression of interleukin-like EMT inducer (ILEI), and EMT itself. We show that poly r(C)-binding protein 1 (PCBP1) binds ILEI transcript and repress its translation. TGF-beta activation leads to phosphorylation at serine-43 of PCBP1 by protein kinase B beta/Akt2, inducing its release from the ILEI transcript and translational activation. Modulation of hnRNP E1 expression modification altered TGF-beta-mediated reversal of translational silencing of ILEI transcripts and EMT. Furthermore, ILEI could induce, as well as maintain, CD24(low)CD44(high) subpopulation in A549 cells treated with TGF-beta, which might explain its capability to induce metastatic progression. These results thus validate the existence of an evolutionary conserved TGF-beta-inducible post-transcriptional regulon that controls EMT and subsequent metastatic progression of lung cancer.
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