期刊
TUMOR BIOLOGY
卷 31, 期 4, 页码 321-331出版社
SAGE PUBLICATIONS LTD
DOI: 10.1007/s13277-010-0040-x
关键词
CpG island methylator phenotype; Sporadic breast cancer; Methylation profile
类别
资金
- Science Funds for Distinguished Young Scholar of Hubei Province [2008154]
- Natural Science Foundation of Hubei Province [2007ABA371]
- Hubei Provincial Department of Education [Q20082407]
- Innovation Foundation for Young Scholars of Yunyang Medical College [CXX200805]
CpG island methylator phenotype (CIMP) involves methylation targeted toward the promoters of multiple genes. We determined a methylation profile of tumor-related genes in serum of sporadic breast cancer (SBC). The multigene methylation was examined by methylation-specific polymerase chain reaction assay in serum of 50 SBCs and 50 paired nontumors, and CIMP+ was defined as having three genes that are concordantly methylated. The methylation frequency of ten genes in serum of 50 SBCs varied from 10% in FHIT to 74% in RASSF1A. The methylation status of RASSF1A, BRCA1, p16, CDH1, ER, RAR beta 2, APC, and DAPK was significantly correlated with SBC and nontumor serum (P<0.05). Methylation of at least one gene was found in 92% SBC; CIMP was more frequent in SBC than nontumor serum (P<0.001). There was a significant association between CIMP and methylation of RASSF1A, BRCA1, p16, CDH1, ER, RAR beta 2, APC, and DAPK (P<0.05); the methylation link profile of CDH1, RASSF1A, BRCA1, and RAR beta 2 as breast cancer marker may contribute high sensitivity (90%) and specificity (88%). ER and RAR beta 2 methylation was associated with elevated serum CA153 levels in 39 SBC samples with CIMP+ (P<0.05). Multivariate analysis showed that living area of patients was found to provide independent prognostic information associated with a relative risk of tumor recurrence of 5.3. Multigene-specific methylation profile in serum was association with the recurrence risk of rural SBC, and positive correlation of CIMP can serve as a promising molecular marker of SBC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据