期刊
TROPICAL ANIMAL HEALTH AND PRODUCTION
卷 43, 期 5, 页码 1049-1055出版社
SPRINGER
DOI: 10.1007/s11250-011-9804-6
关键词
Boar; Frozen semen; Sperm supernatant; Seminal plasma; Semen extender; Motility
资金
- Thailand Research Fund [MRG 4980040, RTA 5080010]
- National Research Council of Thailand (NRCT)
The aim of this study was to evaluate the effect of post-thawing dilution with autologous and heterologous sperm supernatant on motility of frozen-thawed boar spermatozoa. During the cryopreservation, sperm supernatant (a combination of seminal plasma and semen extender, 50% v/v) or seminal plasma from nine boars (Duroc, Large White, and Landrace; three in each) was collected by centrifugation and stored frozen until use as post-thawing solution. Sperm pellets were further processed and cryopreserved using control-rate freezer and was thawed at 50A degrees C for 12 s. After thawing, frozen thawed semen samples were diluted with seminal plasma (group A), supernatant from Landrace (group B), supernatant from Large White (group C), supernatant from Duroc (group D), and Modena (TM) semen extender (group E). Post-thawing motility was evaluated using a phase-contrast microscope after thawing at 1, 10, 20, and 30 min. The present results show that at 1 min, a significantly higher percentage (P a parts per thousand currency signaEuro parts per thousand 0.001) of progressive motility was found in groups B (53.3%) and C (53.9%) than the other groups. At 10 min, the highest (P a parts per thousand currency signaEuro parts per thousand 0.001) progressive motility was found in groups B (65%) and C (61%). At 20 and 30 min, a significantly higher percentage (P a parts per thousand currency signaEuro parts per thousand 0.001) of progressive motility was found in groups B (58.9%), C (53.5%), and D (45.6%) than groups A (3.9%) and E (20.6%). It can be stated that supernatant from the freezing processes (consisting of seminal plasma and Modena (TM), 50% v/v) had a beneficial effect on post-thawing progressive motility of frozen boar semen.
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