期刊
TRENDS IN PLANT SCIENCE
卷 19, 期 3, 页码 183-192出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tplants.2013.12.001
关键词
chemical speciation; plant cells; synchrotron-based techniques
资金
- Natural Science Foundation of China [31372123]
- Innovative Research Team Development Plan of the Ministry of Education of China [IRT1256]
- UK Biotechnology and Biological Sciences Research Council [BB/H006303/1]
- Engineering and Physical Sciences Research Council (EPSRC) [EP/I026584/1]
- Diamond Light Source [SP6042, SP7087]
- BBSRC [BBS/E/C/00005206, BB/H006303/1, BBS/E/C/00004981, BBS/E/C/00005197] Funding Source: UKRI
- EPSRC [EP/I026584/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00005197, BBS/E/C/00004981, BBS/E/C/00004174, BB/H006303/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/I026584/1] Funding Source: researchfish
To maintain cellular homeostasis, concentrations, chemical speciation, and localization of mineral nutrients and toxic trace elements need to be regulated. Imaging the cellular and subcellular localization of elements and measuring their in situ chemical speciation are challenging tasks that can be undertaken using synchrotron-based techniques, such as X-ray fluorescence and X-ray absorption spectrometry, and mass spectrometry-based techniques, such as secondary ion mass spectrometry and laser-ablation inductively coupled plasma mass spectrometry. We review the advantages and limitations of these techniques, and discuss examples of their applications, which have revealed highly heterogeneous distribution patterns of elements in different cell types, often varying in chemical speciation. Combining these techniques with molecular genetic approaches can unravel functions of genes involved in element homeostasis.
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