期刊
TRENDS IN PHARMACOLOGICAL SCIENCES
卷 34, 期 9, 页码 497-507出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tips.2013.07.002
关键词
colorimetric assays; FRET; transferases; hydrolases; matrix metalloproteinases
资金
- Canadian Institutes of Health Research [CIHR-2012 MOP-119425]
- Natural Sciences and Engineering Research Council of Canada [NSERC STPGP/350345-2007]
Optimal enzyme activity is essential for maintenance of physiological homeostasis. A variety of both non-genetic and genetic disruptions can excessively activate or silence intrinsic enzyme activities, with pathological outcomes. Many pharmacological agents are activators and inhibitors of enzymes. It is essential, therefore, in the development of drugs as enzyme activators and inhibitors, that enzyme activities be accurately measured under physiological and pathological conditions. Different biochemical assays have been developed for this purpose, some of which are based on nanostructured materials. This review focuses on gold nanoparticle (GNP)-based structures for the sensing and measurement of enzyme activities in biological specimens. Here we provide an overview and critical analysis of such assays, identify their advantages and limitations, and discuss interesting features of GNPs to be exploited for future applications in pharmacology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据