期刊
TRENDS IN FOOD SCIENCE & TECHNOLOGY
卷 37, 期 2, 页码 115-126出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tifs.2014.03.008
关键词
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资金
- British Food Standard Agency (FSA) through the project GMOseek under the European ERA-NET consortium SAFEFOODERA [G03032]
- British Food Standard Agency (FSA) through the project GMOval [FS244027]
- German Federal Office of Consumer Protection and Food Safety (BVL)
As for many areas of molecular testing, detection of Genetically Modified Organisms (GMO) relies on the real-time Polymerase Chain Reaction (qPCR) technology. Due to the increasing number of GMO, a screening approach using qualitative screening methods has become an integrated part of GMO detection. However, specific guidelines for the validation of these methods are lacking. Here, a pragmatic approach to conduct in-house and inter-laboratory validation studies for GMO screening methods, is proposed. Such guidelines could be adapted to other areas where qualitative qPCR methods are used for molecular testing allowing to implement easily a more reliable screening phase where necessary.
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