期刊
TRENDS IN CELL BIOLOGY
卷 18, 期 9, 页码 443-450出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tcb.2008.07.001
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资金
- Consortium [NIH GM 64346, NIH P01-HL080166]
Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.
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