期刊
TRENDS IN BIOTECHNOLOGY
卷 37, 期 1, 页码 38-55出版社
CELL PRESS
DOI: 10.1016/j.tibtech.2018.08.002
关键词
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资金
- Luxembourg Institute of Health (LIH)
- Fondation Cancer of Luxembourg
- Televie-FNRS [7.6533.16, 7.4592.14]
- Luxembourg National Research Fund (FNR) [C17/BM/11664971/DEMICS]
High-throughput genetic screens interfering with gene expression are invaluable tools to identify gene function and phenotype-to-genotype interactions. Implementing such screens in the laboratory is challenging, and the choice between currently available technologies based on RNAi and CRISPR/Cas9 (CRISPR-associated protein 9) is not trivial. Identifying reliable candidate hits requires a streamlined experimental setup adjusted to the specific biological question. Here, we provide a critical assessment of the various RNAi/CRISPR approaches to pooled screens and discuss their advantages and pitfalls. We specify a set of best practices for key parameters enabling a reproducible screen and provide a detailed overview of analysis methods and repositories for identifying the best candidate gene hits.
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