期刊
TRENDS IN BIOTECHNOLOGY
卷 29, 期 11, 页码 586-594出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2011.06.004
关键词
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资金
- Science Foundation of Ireland [SFI 09/SIRG/B1654]
- Biotechnology and Biological Sciences Research Council (BBSRC)
- East of England Development Agency (EEDA)
- Norfolk County Council
- Greater Norwich Development Partnership
- Norwich City Council and South Norfolk Council
The arrival of second-generation sequencing has revolutionized the study of bacteria within a short period. The sequence information generated from these platforms has helped in our understanding of bacterial development, adaptation and diversity and how bacteria cause disease. Furthermore, these technologies have quickly been adapted for high-throughput studies that were previously performed using DNA cloning or microarray-based applications. This has facilitated a more comprehensive study of bacterial transcriptomes through RNA sequencing (RNA-Seq) and the systematic determination of gene function by 'transposon monitoring'. In this review, we provide an outline of these powerful tools and the in silico analyses used in their application, and also highlight the biological questions being addressed in these approaches.
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