期刊
TRENDS IN BIOTECHNOLOGY
卷 28, 期 6, 页码 291-299出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2010.03.001
关键词
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资金
- Environmental Biotechnology National Core Research Center (KOSEF) [R15-2003-012-02002-0]
- TDPAF (Technology Development Program for Agriculture and Forestry) [NMC0300837]
- CAER (Center for Aquatic Ecosystem Restoration)
- Korea Food & Drug Administration [09172KFDA996]
Recent advances in molecular biology have resulted in the application of DNA microarrays and next-generation sequencing (NGS) technologies to the field of microbial ecology. This review aims to examine the strengths and weaknesses of each of the methodologies, including depth and ease of analysis, throughput and cost-effectiveness. It also intends to highlight the optimal application of each of the individual technologies toward the study of a particular environment and identify potential synergies between the two main technologies, whereby both sample number and coverage can be maximized. We suggest that the efficient use of microarray and NGS technologies will allow researchers to advance the field of microbial ecology, and importantly, improve our understanding of the role of microorganisms in their various environments.
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