期刊
TRENDS IN BIOTECHNOLOGY
卷 26, 期 2, 页码 57-59出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2007.10.010
关键词
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Classical strategies for gene microarrays require labeling of probes or target nucleic acids with signaling molecules, a process that is expensive, time consuming and not always reliable. Bazan and colleagues showed that a nucleic acid-binding cationic conjugated polyelectrolyte can be used in label-free DNA microarrays based on surfaces modified with neutral peptide nucleic acid (PNA) probes. This technique provides a simple and sensitive method for DNA detection without the need for covalent labeling of target DNA.
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