期刊
TRENDS IN BIOCHEMICAL SCIENCES
卷 39, 期 1, 页码 1-7出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibs.2013.11.001
关键词
rate temperature-dependency; thermophilic enzymes; active-site preorganization; enzyme dynamics; thermal vibrations
资金
- Israel Science Foundation
Organismal adaptation to extreme temperatures yields enzymes with distinct configurational stabilities, including thermophilic and psychrophilic enzymes, which are adapted to high and low temperatures, respectively. These enzymes are widely assumed to also have unique rate temperature dependencies. Thermophilic enzymes, for example, are considered optimal at high temperatures and effectively inactive at low temperatures due to excess rigidity. Surveying published data, we find that thermophilic, mesophilic, and psychrophilic enzymes exhibit indistinguishable rate temperature dependencies. Furthermore, given the nonenzymatic rate temperature dependency, all enzymes, regardless of their operation temperatures, become >10-fold less powerful catalysts per 25 C temperature increase. Among other factors, this loss of rate acceleration may be ascribed to thermally induced vibrations compromising the activesite catalytic configuration, suggesting that many enzymes are in fact insufficiently rigid.
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